Signalling

Part:BBa_K2833000:Design

Designed by: CHENG BOXUAN   Group: iGEM18_BJRS_China   (2018-10-03)


J23104+B0032+sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 75


Design Notes

We used this part to test the expression strength of J23104 combined with B0032


Source

We acquired the sfGFP gene from Chen Jianguo Lab.

References